1. animal science    2. biotechnology   3. clone pig

1. Chinese Society of Animal Science

2. The Chinese Society of Laboratory Animal Science

3. International Xenotransplantation Association

1. Tu, C. F., K. B. Choo and W. T. K. Cheng. 1995. Production and analysis of trangenic pig with human factor IX gene. J. Chin. Soc. Anim. Sci. 24: 273-287. (in Chinese)

2. Tu, C. F., R. C. S. Ma and W. T. K. Cheng. 1998. Effect of operation tme on pronuclear embryos recovery rate and relative centrifugal field on pronucleus-exposured ratio and embryonic survival rate. J. Chin. Soc. Anim. Sci. 27: 49-58. (in Chinese)

3. Tu, C. F., T. Sato, M. Hagihara, K. H. Lee, Y. C. Lee, C. N. Weng, R. Chu, K. Tsuji, C. J. Lee. 1998. The expression of HLA-DP antigen on peripheral blood mononuclear cells of HLA-DP transgenic pigs. Transplant. Proc. 30 (7A): 3502-3.

4.  Tu, C. F., L. H. Hwang and W. T. K. Cheng. 1999. Generation of transgenic mice by subzonal infection methods after microinjection of the retroviral particles into perivitelline space of mouse early embryos. J. Chin. Soc. Anim. Sci. 28: 381-394. (in Chinese)

5. Tu, C. F. K. Tsuji, K. H. Lee, R. Chu, T. J. Sun, Y. C. Lee, C. N. Weng, C. J. Lee. 1999. Generation of HLA-DP transgenic pigs for the study of xenotransplantation. Int. Surg. 84:176-182.

6. Huang, H. W., T. Z. Liu. K. H. Lee, C. F. Tu, W. C. Lee, T. Shimogiri, H. Mannen  and S. S. O. Li. 2000. cDNA cloning of pig testicular lactate dehydrogenase-C, thermal stablility of the expressed enzyme, and polymorphism among strains. Gene 242:151-154.

7. Tu, C. Fu., S. L. Hsieh, J. M. Lee, L. L.Yang, T. Sato, K. H. Lee, C. N. Weng, S. J. Mao, K. Tsuji, C. J. Lee. 2000. Successful generation of transgenic pigs for human decay-accelerating factor and human leucocyte antigen DQ. Transplant Proc. 32(5): 913-5. (SCI; NSC87-2313-B-059-014-, and NSC88-2313-B-059 -011 A 21)

8. King, Y. T., W. C. Lee, M. S. Gao, J. L. Wang, C. F. Tu, S. C. Wu and Y. H. Kuo. 2000. Synthesis of 60- and 72-kDa heat shock proteins in early stage of porcine embryogenesis. Anim. Reprod Sci. 63: 221-229. (SCI)

9. Lee, J. M., C. F. Tu, K. Tsuji, Y. C. Lee, C. J. Lee. 2000. The effective antigen presentation of human MHC on the lymphocyte of HLA DPw4 transgenic pig: examination with xenogenic mixed lymphocyte culture and primed lymphocyte test. Transplant. Proc.32: 2503-2504. (SCI)

10. Hsu, P. N., H. H. Lin, C. F. Tu, N. J. Chen, K. M. Wu, H. F. Tsai, S. L. Hsieh. 2001. Human FasL gene does not induce inflammation in pancreas but is unable to protect islet graft from transplantation rejection in pancreas-specific hFasL transgenic mice. J. Biol. Med. 8: 262-269. (SCI; NSC89-2313-B-059-027-A21)

11. Tu, C. F., S. C. Wu, P. C. Yang and W. T. K. Cheng. 2001. Technology and application of transgenesis in livestock. J. Chin. Soc. Anim. Sci. 30: 37-43. (in Chinese)

12. Tu, C. F. and L. L. Yang. 2001. Generation of transgenic mice carrying HLA-DR (A1+B1) and CMV-hDAF three transgenes. J. Chin. Soc. Anim. Sci. 30: 129-140. (in Chinese)

13.  Lee, J. M..,# C. F. Tu,# P. W. Yang, K. H. Lee, K. Tsuji, M. K. Tsai, R. J. Chen, C. Y. Hu, R. P. Hsieh, H. C. Tai, B. L Chiang, C. N. Weng, Y. C. Lee, C. J. Lee. 2002. Reduction of human-to-pig cellular response by alteration of porcine MHC with human HLA DPw0401 exogenes. Transplantation 73(2): 193-197. (SCI;) (equal contribution)

14. Juang, J. J., C. F. Tu, C. H. Kuo, P. N. Hsu. 2002. No protective effect of Fas-ligand transgenic islet on allogeneic rejection. Transplant. Proc. 34: 1456-1457. (SCI)

15. Tu, C. Fu., J. M. Lee, T. Sato, H. C. Tai^., F. R. Lee, C. K. Yang, K. Tsuji^., C. J. Lee. 2003. Expression of HLA-DQ in transgenic pigs. Transplant. Proc. 35: 513-515. 

16. Tu, C. F., M. S. Liu and Y. H. Kuo. 2003. A simple vitrification procedure for cryopreservation of transgenic mouse embryos. J. Chin. Soc. Anim. Sci. 32: 33-42. (in Chinese)

17. Lee, K. H., C. F. Tu, S. C. Wu and W. C. Hsu. 2003. Routine generation of green fluorescent chimeric mice with a high percentage of germline transmission by a novel embryonic stem cell line. J. Chin. Soc. Anim. Sci. 32(2): 143-154. (in Chinese)

18. Tu, C. F., K. F. Huang, I. C. Chen, C. J. Lin, S. C. Wu and P. C. Yang. 2004. Application of real time PCR to rapidly screen hirudin transgenic piglets. J. Chin. Soc. Anim. Sci. 33(4): 1-12.  (in Chinese)

19. Lee, J. M., C. F. Tu, S. C. Huang, K. Tsuji, R. J. Chen, C. Y. Hu, R. P. Hsieh, H. C. Dai, C. N. Weng, Y. C. Lee, C. J. Lee. 2003. Attenuation of human–to-pig xenogenic cellular proliferation and Th1 response by expressing the human MHC II DQ exogenes. On porcine cells. Transplant. Proc. 35: 527-528. (SCI).

20. Lee, J. W., S. C. Wu, C. Tian, M. Barber, T. Hoagland, J. Riesen, K. H. Lee, C. F. Tu, W. T. Cheng, X. Yang. 2003. Production of cloned pigs by whole cell intracytoplasmic microinjection. Biol. Reprod. 69(3): 995-1001.

21. Wang, B. T., C. F. Tu, L. J.Hsieh, H. C. Tai, Y. L. Chiu, J. M. Lee, S. J. Kuo, K. Tsuji and C. J. Lee*. 2004. Detection of HLA transgenic pigs by fluorescence in situ hybridization for future human xenotransplantation. Xenotransplantation 11: 471-475.

22. Huang, S. Y., C. F. Tu, S. H. Leu, y. h. kuo. 2004. Motility and fertility of alginate encapsulated boar spermatozoa. Anim. Reprod. Sci (in press).

23. Chen, M. Y., C. F. Tu, S. Y. Huang, J. H. Lin, B. S. Tzang, H. L. Tsou, T. H. Hseu, W. C. Lee*. 2005. Augmenting thermotolerance in primary skin fibroblasts from a transgenic pig with overexpressed porcine HSP70.2. Asian-Australia J. Anim. Sci. 18(1):107-112.

24. Tu, C. F., C. H. Kuo, J. H. Juang*. 2005. Effects of heme oxygenase-1 transgenic islets on transplantation. transplant. proc. 37:3463-3467. (SCI)【所外合作論文】

25. Huang, S. Y., Y. H. Chen, S. H. Teng, I. C. Chen, L. L. Ho and C. F. Tu.*, 2006. Protein expression of lymphocytes in HLA-DR transgenic pigs by a proteomic approach. Proteomics 6(21):5815-5825. (SCI, IF=6.088)

26. Lee, J. M., C. F. Tu, H. C. Tai, N. K. Chou, T. S. Yang, C. N. Weng, Y. C. Lee and P. H. Lee*. 2006. The hDAF exogene protects swine endothelial and peripheral blood mononuclear cells from xenoreactive antibody mediated cytotoxicity in hDAF transgenic pigs. Transplant. Proc. 38(7):2270-2272.

27. Lee K. H.*, C. k. Chuang^., H. W. Wang, L. Stone, C. H. Chen, C. F. Tu. 2006. Efficient and mass production of murine chimeric embryos by coculturing denuded embryos and embryonic stem cells in Eppendorf vials. Theriogenology. 67:228-237.

28. 戴浩志、李章銘、周迺寬、杜清富、李伯皇。2007。異種移植：以乳糖轉移酶基因剔除豬為例。台灣醫學11(1)：70-78。

29. 杜清富。2007。誰說人面豬心不可能？科技加持基因轉殖豬加值。科學月刊。447 (3):189-193。

30. Chen, C. H., L. Stone, J. C. Ju, W. T. Lien, M. S. Liu, C. F. Tu and K. H. Lee*. 2007. Generation of cloned mice expressing enhanced green fluorescent protein by activation stimuli combined with 6-dimethylaminopurine. Reprod. Dom. Anim. (In press)

31. Lee, J. M.., C. F. Tu, H. C. Tai, N. K. Chou, C. N. Weng, Y. C. Lee, C. J. Lee, P. H. Lee. 2008. The impacts of human ABO blood groups on human xenoreactive antibody-mediated cytotoxicity and the protective effect of human decay accelerating factors (hDAF) exogene on the swine endothelium. Transplant Proc (In press).

32. Tu, C. F.*, H. C. Tai, C. m. Chen, T. T. Hung, J. M. Lee, T. S. Yang, C. H. Chen, Y. L. Tseng, N. K. Chou, P. H. Lee. 2008. Human Leukocyte Antigen-DR Matching Improved Skin Graft From Ttansgenic Pigs to Accommodate in SCID Mice Reconstructed with Human Peripheral Blood Mononuclear Cells. Transplant Proc (In press).

33. Tai, H. C., C. F. Tu, L. L. Ho, Y. C. Chao, N. K. Chou, J. M. Lee, T. S. Yuang, P. H. Lee, Y. B. Tang, K. J. 2008. Long-Term Survival of HLA-DR Pig Skin in SCID Mice after Reconstitution with Human PBMC and Short-Term Immunosuppression. Transplant Proc (In press).

34. Yen C. H., C. K. Yang, C. S. Lin, I. C. Chen and C. F. Tu*. 2008. Expression the synthetic Hirudin genes in transgenic Animals milk driven by mammary-specific goat b-casein sequences. Biotech. J. (Accepted).

35. 杜清富、戴浩志、楊天樹、李章銘、曾堯麟、周迺寬、楊卿堯、莊景凱、翁仲男、李伯皇。2008。豬隻基因工程發展做為異種移植研究。台灣醫學。(Accepted)

36. Huang, S. Y., J. H. Lin, Y. H. Chen, Shih-Hua Teng, W. T. K. Cheng, S. C. Wu* and C. F. Tu*. 2008. Comparison of Protein Expression in the Mammary Gland from Different Lines of Human Factor IX Transgenic and Non-transgenic Pigs Using a Proteomic Approach. Proteomics (Submitted).

1. Tu, C. F., C. J. Lee, J. M. Lee, K. Tsuj. 2003. Transgenic swine having HLA-D gene, swine cells thereof and xenografts there from. (US 6,639,122 B1)(專利期間：92.10.28-109.9.19).

2. Tu, C. F., C. K. Yang, C. S. Lin, C. H. Yen, C. H. Chen, S. C. Wu. Y. L. Sun, M. S. Leu, P. C. Yang, 2003. Expression vector for hirudin and transformed cells and transgenic animals containing said vector. (USA patent Application . No. 10/727,145) (CIP)

3. Tzang, B. S., C. F. Tu, W. C. Lee, M. Y Chen, S. Y. Huang, J. H. Lin. Transgenic pig containing heat shock protein 70 transgene. (USA patent Application No. 10/350.354) (CIP)( US 7,115,796) (專利期間：95.10.3-112.10.9)。

4. Lee, K. H., H. W. Hang, H. R. Chang, C. F. Tu, C. J. Lin. 2004. A Method for generating non-human mammalian chimeric embryo.(USA patent Application No. 10/875,225)

5. 李坤雄、王慧雯、張惠溶、杜清富、林之任。2004。產製非人類之哺乳動物嵌合胚的方法。(中華民國專利申請中)。案號：92136457。

6. 李坤雄、王慧雯、張惠溶、杜清富、林之任。2004。產製非人類之哺乳動物嵌合胚的方法。(大陸專利申請中)。案號：200410000197.5。  

7. Tu C. F., C. K. Yang, M. S. Leu, L. L. Ho, K. F. Huang, H. C. Tai and C. J. Lee. 2004. Transgenic pigs carrying both hHO-1 and hDAF transgenes for xenotransplantation. (USA patent Application No. 10/857,613)

8. 李坤雄、張惠溶、杜清富。2005。小屬胚幹細胞株。(中華民國專利)。案號：091125394，証號第229131。專利期間(94.3.11-111.10.24)。

9. 顏重河、林寅申、杜清富、陳逸仲。2005。自基因轉殖動物乳汁中分離水蛭素之方法。(中華民國專利申請中)。案號：94138861。

10. 李坤雄、陳建宏、石力、杜清富。2005。產製非人類之哺乳動物核移置胚的方法。(中華民國專利申請中)。案號：94146612。

1. Tu, C. F. 2000. Biotech and pigs production. Pig Research Institute Taiwan Ed.: The leaders of pig production in 21st centuries. Pp 10-13. Pig Research Institute Taiwan, Chunan, Miaoli.

2. 鄭登貴、杜清富、吳信志， 2000。生物技術與畜牧產業。現代學術研究-環保與科技  pp.153-178。現代學術研究基金會。台北。(ISBN 957-99963-6-9)

3. 杜清富。2003。利用反轉錄病毒載體法產製基因轉殖動物。動物基因轉殖技術與實驗  pp58-70。教育部顧問室「生物技術科技教育改進計畫」。(ISNB 957-01-7605-9)

4. 杜清富、鄭登貴。2003。基因轉殖豬之產製。動物基因轉殖技術與實驗  pp172-188。教育部顧問室「生物技術科技教育改進計畫」。(ISNB 957-01-7605-9)

5. 杜清富。2004。「基因轉殖家畜禽」生產醫藥蛋白質。科技報導  270(6)：16-19。  

6. 杜清富。2004。國內外家畜禽基因轉殖科技之應用與發展。農科新世紀-農業生物科技園區專訊  1: 36-40。

7. 杜清富。2004。生命的延續-異種移植。「生命科技與生命倫理」。玄奘大學。  

8. 杜清富。2005。豬器官供人類異種移植使用之展望。農科新世紀-農業生物科技園區專訊。3:28-31。

9. 杜清富、劉學陶。2005。基因轉殖家畜禽隔離田間試驗場之介紹。農業生技產業季刊。2:52-56。

10. 杜清富、劉學陶、曾勝義。2007。因轉殖家畜禽隔離田間試驗場，品質手冊，ISO9001認證一階文件。

11. 劉學陶、曾勝義、杜清富。2007。因轉殖家畜禽隔離田間試驗場，品保程序書 ，ISO9001認證二階文件。

12. 劉學陶、曾勝義、陳啟銘、杜清富。2007。基因轉殖家畜禽隔離田間試驗場，標準操作步驟 ，ISO9001認證三階及四階文件。

13. 杜清富、方文德、顏重河、楊程堯、楊啟裕、張有勝、周佑吉、王仕蓉。2008。以基因轉殖豬產製人類第九凝血因子技術平台之建立與安全功效評估。經濟部：創新深耕生技研發成果產業化季刊。P10-16。